FPGG016 - Magnetic and Ultra-sounds living tissue Micromanipulation Mimicking and Monitoring Tumour Growth Pressure in vivo
Increasing evidences show the involvement of mechano-transduction processes in the regulation of the interplay between shape-related strains and state of expression of the genome in living tissues. However, tools allowing long term control of physiological mechanical strains from the inside of the tissues are lacking to investigate the impact of such processes in organisms in vivo. We recently found ex-vivo that pressure, potentially associated to intestinal transit or tumour growth, triggers the activation of the
primary oncogene program in genetically predisposed pre-tumoral APC+/- mice colon tissues. Here we will investigate in vivo the involvement of such mechano-transduction process leading to oncogene expression, in response to local pressure mimicking tumour growth strains applied to the surrounding pretumoral tissue. This interdisciplinary project is based on the development of new experimental physical techniques allowing to mimic soft tumour pressure increase, based on the manipulation of magnetic fluid
locally concentrated into the cells by natural ways and endocytosis, and stable on the months time scale, coupled to acoustic method allowing both the monitoring of the ferro-fluid position and pressure cytodynamics in vivo, and the remote generation of local mechanical strains induced by the ultrasonic beam radiation force to explore the response of the tissue to short time scale perturbation.
Laboratory of Mechanics and Genetics of Embryonic and Tumoral Development - Institut Curie
Main Activity : Biology / Physics
Emmanuel Farge
Laboratory of Electrolytes & Colloids Physical Chemistry and Analytical Sciences (PESCA) - ESPCI
Main Activity : Chemistry / Physics
Christine Ménager & Valérie Cabuil
"Wave Physics For Medicine" Institut Langevin - ESPCI
Main Activity : Acoustics / Medicals
Mickael Tanter